5-CFDA, AM [5-Carboxyfluorescein Diacetate, Acetoxymethyl Ester]

5-Carboxyfluorescein diacetate, acetoxymethyl ester (CFDA, AM) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is required to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product.